Our Scientific Solutions

Gut On Chip

innovation radar category winner react4life

Gut On Chip: Nutraceutical Food & Feed

Gut on chip is our solution to study the physiological and pathological environment of the gut, the effects of active compounds on intestinal permeability and gut barrier function, as well as support in dose definition and mechanistic studies.
Our Gut on Chip technology, combined with a monolayer of cells or reconstructed human oral epithelial tissue, is a valuable in vitro tool for studying molecular transport under highly predictive conditions that closely resemble the dynamic in vivo environment.

Compared to traditional in vitro tests carried out in static conditions, our technology more faithfully reproduces the physiological environment, allowing for more accurate pre-screening of molecules and safety tests.
This represents a new and effective way to test the bioavailability and efficacy of new molecules: more predictive than standard in vitro studies, faster and cheaper than in vivo tests.

Gut on Chip Applications

Claim Substantiation

To objectivate, validate and support product claims with scientific evidence through precise, reliable and reproducible testing on humanized models with gut on chip.

Efficacy Testing

To test the efficacy of active ingredients or formulations for dietary supplements or functional foods by studying how they interact with human gut cells.

Mechanistic Insights

To gain a deep understanding of how bioactive molecules function in human body, studying their pathways and effects at a cellular level.

Pre-screening

To screen and select the most promising molecules before animal or human testing, offering valuable data for product development while saving time and costs.

Absorption Testing

To study how molecules act from the oral administration through the gastric and digestive phases, providing valuable data for product development.

Toxicity Testing

To identify potentially harmful effects on specific organs, including evaluating cytotoxicity from the dosage of natural ingredients, mitigating risks.

Gut on Chip: Gut-organ axis

Gut on chip organ axis react4life nutraceutical

Example of gut on chip assays

ADME STUDIES
NUTRIENTS UPTAKE
MICROBIOME RESEARCH
LEAKY GUT ASSAY
MOLECULE SCREENING

BIOAVAILABILITY
INFLAMMATION
TOXICITY
PROBIOTICS ADHESION
POSTBIOTIC EFFICACY

POSSIBLE READOUTS

Gut on Chip

ABSORPTION KINETICS

CYTOTOXICITY

EFFICACY ASSAY

Gut on chip supports not just a single type of quantitative measurement, but enables multiple parallel measurements at analytical, biological, and molecular levels.

gut on chip inflammation cells millifluidic react4life

INFLAMMATORY RESPONSE

BARRIER INTEGRITY

COMPLETE DIGESTION

Gut on Chip: References

Marrella A et al, Altex 2020 – In vitro demonstration of intestinal absorption mechanisms of different sugars using 3D organotypic tissues in a fluidic device. Read now >

Fedi A et al, Journal of Controlled Release 2021 – In vitro models replicating the human intestinal epithelium for absorption and metabolism studies: A systematic review. Read now >

L’Integratore Nutrizionale 2020/23 – Sviluppo e validazione di una piattaforma innovativa per lo studio in vitro dei diversi meccanismi di assorbimento intestinal. Read now >