Leaky Gut Assay

The integrity of the intestinal barrier is crucial for maintaining overall gut health, preventing harmful
substances from entering the bloodstream. When this barrier is compromised, a condition known as “leaky gut” occurs, which is associated with gastrointestinal disorders and systemic inflammation.

Purpose of the Test

This assay evaluates the regenerative potential of a test substance by tailoring an advanced human leaky gut in vitro model. By creating a controlled wound in the gut
cells monolayer, the assay measures wound closure rates and barrier function over time, providing valuable insights into the substance’s ability to restore epithelial integrity and promote gut barrier repair.

This assay is generally used as a standard in preclinical studies to support the safety, efficacy, and therapeutic claim of a product.

The MIVO® Advantage: Accelerating Epithelial Repair Insights

The MIVO® platform offers a groundbreaking approach to studying leaky gut by simulating both luminal and blood flow in a dynamic, human-relevant gut environment. Unlike traditional static models, which fail to replicate the complex physiological conditions of the human gut, MIVO® advanced organ-on-chip system allows for more accurate assessment of epithelial barrier restoration and gut permeability in real-time. This innovative model enables researchers to evaluate the ability of test substances to repair leaky gut more effectively, providing a predictive tool for understanding therapeutic efficacy.

Driving Innovation and Setting New Standards

While regulatory frameworks for probiotic testing in gut health are still evolving, the MIVO® platform positions research at the forefront of this emerging field, paving the way for a new era in microbiome research. This innovative approach challenges the status quo, providing critical data that can drive further scientific exploration and potentially shape future regulatory standards in probiotic and microbiome-based therapies.

leaky gut assay

Our Unique Value Proposition

Realistic, Humanized Model: The MIVO® platform integrates dynamic fluid flow to accurately simulate the physiological conditions of the human gut. This provides a more authentic environment for studying epithelial repair and regeneration compared to static models.
Dynamic Fluid Flow for Enhanced Precision: Unlike traditional assays, MIVO® double-flow system replicates both luminal and blood flow, creating a highly realistic setting for gut wound healing studies. This dynamic environment significantly improves the accuracy of barrier integrity, intestinal permeability, and wound closure analyses.
Comprehensive and Predictive Assessment: By enabling a detailed investigation of epithelial repair, tight junction formation, and gut barrier integrity, the MIVO® platform provides a robust solution for testing the efficacy of treatments targeting leaky gut. Its versatility makes it ideal for assessing a wide variety of compounds, from pharmaceuticals to nutraceuticals, offering a valuable tool for both therapeutic and product development across multiple sectors.

Leaky Gut Assay

In summary

Leaky gut assay tab
3D CoCulture

READOUTS

  • Epithelial barrier integrity (TEER measurement)
  • Tight junctions specific staining
  • FITC-dextran permeability
  • Inflammation (i.e. IL6, IL8)
TEER measurement is performed before and after exposure to test drug, as indirect measurement of barrier integrity leaky

TEER measurement is performed before and after exposure to test drug, as indirect measurement of barrier integrity.

FITC-dextran permeability assay is performed to measure membrane integrity leaky gut assay

FITC-dextran permeability assay is performed to measure membrane integrity .

The ability of testing formulation to induce gut inflammation is assessed by quantification of pro-inflammatory cytokines (i.e. IL6, IL8) through ELISA assay leaky

The ability of testing formulation to induce gut inflammation is assessed by quantification of pro-inflammatory cytokines (i.e. IL6, IL8) through ELISA
assay.

ZO-1 immunostaining is performed to investigate the effect of testing compound on tight junctions Drug induced toxicity assay

ZO-1 immunostaining is performed to investigate the effect of testing compound on tight junctions.

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