GUT Permeability Assay

The intestinal permeability assay is a pivotal in vitro cell based assay to assess the ability of a substance to permeate across intestinal epithelium. It is commonly employed to estimate the absorption of orally administered drugs, and to predict their bioavailability.

Purpose of the Test

Gut permeation assay evaluates the intestinal absorption and permability of drugs and substances after their oral administration. It is essential for determining the fraction absorbed and predicting in vivo bioavailability. It utilizes an advanced in vitro fluid dynamic gut model to mimic the intestinal barrier, and measure the apparent permeability coefficient (Papp) to classify compounds as highly or poorly permeable.

Regulatory Compliance

The assay is aligned with international guidelines to provide evidence for drug absorption and support regulatory submissions, according to the BCS guidelines established by regulatory bodies like the FDA and EMA.

The MIVO® Advantage: A Superior Predictive Model

MIVO® gut on chip technology offers a superior model for evaluating intestinal permeability by closely mimicking the physiological conditions of the human intestine, compared to static caco2 cell based assay. The dynamic flow platform closely mimics lumen and circulatory flow conditions, providing superior accuracy in evaluating gut permeability.

Our Unique Value Proposition

Dynamic Fluid Flow for Unmatched Accuracy: In contrast to traditional static models, our unique Gut-on-Chip design introduces a dual fluid flow, simulating both blood and luminal conditions, offering a more realistic model for precise drug absorption evaluation.
PDMS-Free Innovation: MIVO® platform is entirely PDMS-free, ensuring reliable permeability measurements, interference-free results that better reflect the true behavior of test substances.
Comprehensive Assessment of Complex Substances: MIVO® excels in testing a wide range of substances, from small molecules to complex formulations, with variyng physico-chemical properties and solubility, making it ideal for assessing pharmaceuticals, nutraceuticals, food ingredients, across multiple industries.

Gut Absorption Assay

In summary

Gut absorption assay

READOUTS

  • Analytical quantification of kinetics of the fraction of moleculesabsorbed
  • Measure of apparent permeability coefficient (Papp)
Gut absorption analytical quantification

Analytical quantification is performed at
different time-points to assess the drug
permeation over-time.

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