GUT Anti-inflammatory Assay

Intestinal inflammation, particularly chronic conditions like Inflammatory Bowel Disease (IBD), represents
a complex and debilitating issue characterized by persistent inflammation that disrupts gut function.
Studying chronic intestinal inflammation in a controlled, in vitro environment is not only essential for
testing new therapies but also for gaining deeper insights into disease mechanisms. This approach
enables the development of targeted treatments and facilitates a better understanding of disease
progression, ultimately improving patient outcomes.

Purpose of the Test

Anti-inflammatory intestinal assay evaluates the impact of treatments on intestinal tissue functionality and healthy state, with a focus on inflammation recovery, by using a dynamic multicellular intestinal 3D model. This test ensures accurate, reliable, and quantitative data about gastrointestinal therapies efficacy.

The MIVO® Advantage: A Superior Predictive Model

Despite static and traditional assays, this MIVO® based assay supports long-term culture of human intestinal tissues, making it ideal for studying both acute and chronic inflammation. The dynamic flow platform closely mimics lumen and circulatory flow conditions, providing superior accuracy in evaluating treatment effects on gut inflammation over time.

gut inflammation long term

Our Unique Value Proposition

Realistic & Predictive Model: The MIVO® platform provides a dynamic and highly accurate simulation of human gut conditions in both the gut lumen and in circulatory circuit, allowing the study of chronic intestinal inflammation, in a realistic and predictive environment.
Long-Term Culture for Chronic Inflammation: Unlike conventional models, MIVO® gut on chip platform supports long-term culture of gut tissues, enabling the study of chronic inflammation over extended periods and the application of repeated doses of treatment. This feature is critical for understanding the prolonged effects of treatments and the complexities of chronic conditions such as IBD.
Precise Evaluation of Therapeutic Effects: The test offers a controlled environment for evaluating the efficacy of treatments, providing clear insights into their impact on inflammation and gut health.
Accelerating Drug Development: By providing reliable, high-quality data, this test helps researchers and pharmaceutical companies fast-track the development of new treatments for gastrointestinal diseases.

Anti-inflammatory Assay

In summary

anti-inflammatory assay summary
3D CoCulture

READOUTS

  • Epithelial barrier integrity (TEER measurement)
  • Tight junctions specific staining
  • FITC-dextran permeability
  • Inflammation (i.e. IL6, IL8)
anti-inflammatory assay barrier integrity teer

TEER measurement is performed before and after exposure to inflammatory stress, as indirect measurement of barrier integrity.

anti-inflammatory assay fitc dextran permeability

FITC-dextran permeability assay is performed to measure membrane integrity .

ZO-1 immunostaining is performed to investigate the effect of gut inflammation on tight junctions.

anti-inflammatory assay IL8 total release

The ability of testing formulation to ameliorate gut inflammation is assessed by quantification of pro-inflammatory cytokines (i.e. IL6, IL8) through ELISA assay.

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