Gastroprotection Activity

Gastroesophageal reflux is a common disorder that can lead to esophageal irritation and inflammation due to prolonged acid exposure. In vitro testing is essential for evaluating the effectiveness of gastroprotective agents, and exploring their potential in preventing or alleviating esophageal damage prior to clinical application. This test plays a pivotal role in the safety and efficacy assessment of pharmaceutical drugs, natural compounds, and dietary supplements designed for gastroprotection.

Purpose of the Test

The MIVO-based Gastroprotection activity assay uses the MIVO® Gut-on-Chip platform to evaluate the efficacy and safety of gastroprotective agents in a more reliable and human-relevant manner than traditional in vitro models. MIVO Gut-on-Chip platform ®’s ability to faithfully recreate the complex dynamic fluid-flow structure of the human esophagus, along with its compatibility with Reconstructed Human Esophageal (RhE) tissue models, allows for a more accurate prediction of responses to acid reflux.

The MIVO® Advantage: A Superior Predictive Model

MIVO® organ-on-chip technology significantly improves the survival and barrier function of the traditional RhE models (Marrella et al. 2020), offering a more reliable simulation of acid reflux conditions. This advanced testing methodology leverages a multiparametric approach to predict the efficacy of testing compound, providing critical insights into its potential to prevent or alleviate esophageal damage induced by acidic insults.

gastroprotection assay mivo technology organ on chip

Our Unique Value Proposition

Realistic, 3D, Humanized tissue Model: The MIVO® platform integrates a 3D RhE with dynamic fluid flow, closely mimicking real-life conditions of acid reflux for more accurate predictions of esophageal irritation and damage.
Dynamic Fluid Flow for Enhanced Accuracy: Unlike traditional static models, our assay incorporates dynamic fluid flow, replicating the physiological conditions of acid reflux. This dynamic interaction provides more precise and reliable insights into the protective effects of substances on the esophageal tissue.
Comprehensive Evaluation: The test offers a multiparametric approach, including transepithelial electrical resistance (TEER), permeability assays, and cytokine release quantification, ensuring a thorough assessment of the protective capacity of compounds against acid-induced esophageal damage.
Versatile Applications: This test is ideal for evaluating the efficacy of both pharmaceutical drugs and natural compounds, including dietary supplements, making it a versatile tool for safety and efficacy testing across multiple industries.

Gastroprotection Assay

In summary

gastroprotection assay

READOUTS

  • pH monitoring, to check the buffet activity of teh testing compounds
  • Epithelial barrier integrity (TEER measurement) along the acid insult and testing compound treatment
  • FITC-dextran permeability after the treatment
  • Histology for assessing the safety of the treatment
  • Inflammation (i.e. IL6, IL8)

The ability of gastroprotector to ameliorate the inflammation induced by acidic insult is assessed by quantification of pro-inflammatory cytokines.

TEER measurement is performed before and after exposure to acidic stress, as indirect measurement of barrier integrity.

FITC-dextran permeability assay is performed to measure membrane integrity. 

Morphological assessments is conducted by histological analysis, to assess cellular degeneration, erosions and necrosis after acid insult.

Soothing effect is monitored by evaluating the expression of muco-protective markers.

pH levels are measured following the application of acidic insult in presence or absence of the gastroprotector.

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